Elisabeth Ersvær

• Stenseth, Else Berit; Østby, Runa Berg; Ersvær, Elisabeth; Kjøbli, Eli; Kvissel, Anne Katrine & Møller, Ina [Vis alle 8 forfattere av denne artikkelen] (2026). Praksisveiledning: Bruk av case i ekstern praksis. Bioingeniøren. ISSN 0801-6828. 1, s. 22–27. doi: https:/forskning.bioingenioren.no/originalartikkel/praksisveiledning-bruk-av-case-i-ekstern-praksis/144939. Fulltekst i vitenarkiv Vis sammendrag

  Innledning: Studien undersøker bruken av casebasert læring som en metode i praksisveiledning. Case er definert som bioingeniørfaglig relevante scenarioer brukt i teoretisk eller praktisk veiledning. Studien hadde som mål å kartlegge i hvilken grad case brukes i ekstern praksis, hvordan de utarbeides og anvendes på tvers av fagområder og læringssituasjoner, samt å kartlegge praksisveiledernes syn på fordeler og ulemper ved bruk av case. Metode: En deskriptiv metode med både kvantitative og kvalitative tilnærminger ble benyttet. Metoden er basert på en spørreundersøkelse sendt til praksisveiledere i norske sykehus i perioden juni 2023 til januar 2024. Det var totalt 51 respondenter. Resultat: Studien viser at 55 % av praksisveilederne som svarte på spørreundersøkelsen bruker case i veiledningen. Casene er hovedsakelig selvutviklede og basert på problemstillinger fra laboratoriehverdagen. Fra veiledernes perspektiv fremmer casebasert læring studentenes kritiske tenkning, selvstendighet og evne til å integrere teori og praksis. Blant veiledere som i dag ikke benytter case, uttrykte mange interesse for å implementere case i veiledningsarbeidet, samt å samarbeide med kolleger om å utvikle slike caser. Konklusjon: Studien fremhever potensialet casebasert læring har til å gjøre praksisperioden mer engasjerende, fremme dybdelæring og til å kunne tilpasses til studentenes individuelle behov.

• Stenseth, Else Berit; Syversen, Linda; Andersen, Heidi; Wergeland, Line; Granerud, Beathe Kiland & Ersvær, Elisabeth (2025). Ekstern praksis: Samarbeid og kommunikasjon for kvalitet. Bioingeniøren. ISSN 0801-6828. 8 2025, s. 20–25. Fulltekst i vitenarkiv Vis sammendrag

  Bakgrunn: Eksterne praksisstudier er viktig for studentens profesjonsutvikling. Høy kvalitet på praksisstudiene forutsetter at kontakten mellom praksisstedet og utdanningen er systematisk etablert. I denne studien har vi gjennomført en kartlegging av kommunikasjonsmønstre, informasjonsflyt og samarbeid mellom utdanningsinstitusjon og praksisfeltet, sett fra perspektivet til tre bioingeniørutdanninger i Norge. Material og metode: Det er utført fokusgruppeintervju av ansatte ved tre ulike bioingeniørutdanninger og ett sykehus, etterfulgt av tematisk kvalitativ innholdsanalyse av datamaterialet. Resultat: Undersøkelsen av informantenes erfaringer viser (i) manglende avtaler på studieprogram-/instituttnivå, (ii) informasjonsflyt i utdanningsinstitusjonen kan være ulik, muligens grunnet ulik fakultetstilhørighet, (iii) kommunikasjonen mellom utdanning og praksisfelt er kompleks og foregår på ulike nivåer og med ulike kommunikasjonsmønstre, og (iv) det kommuniseres både om fordeling av studenter samt tematisk innhold i praksis. Konklusjon: Denne studien viser at det er variasjon i kommunikasjon fra sted til sted, og at det er mangelfull kommunikasjon både internt i utdanningsinstitusjonene og mellom utdanningsinstitusjon og praksissted.

• Frøland, Tord Waage; Ersvær, Elisabeth & Heldal, Ilona (2025). Tasks-Interactions-Environment for Simulations and Serious Games (TIE-SSG) Framework: Exploring Practical Applications. Proceedings of the European Conference on Games Based Learning (ECGBL). ISSN 2049-0992. 19(2), s. 929–940. doi: 10.34190/ecgbl.19.2.4221. Fulltekst i vitenarkiv Vis sammendrag

  Simulations and Serious Games (SSGs) are increasingly recognized as effective and flexible tools for supportingeducation. Despite their potential, selecting and appropriately using SSGs within learning pathways remains a significantchallenge. To address this, the Task-Interaction-Environment for Simulations and Serious Games (TIE-SSG) framework wasdeveloped. This framework is designed to evaluate and integrate SSGs based on their fidelity levels, while adhering toinstructional scaffolding principles to achieve specific learning goals (Frøland et al., 2025). This paper introduces strategiesfor utilizing the TIE-SSG framework to evaluate and seamlessly integrate SSGs into educational curricula, particularly forhealthcare education. It outlines a step-by-step guideline that emphasizes three key phases: (1) prerequisites, (2)assessment, and (3) integration. Supporting tools and templates are provided to standardize evaluations and facilitate thealignment of SSGs with learning objectives. The practical application of the TIE-SSG framework is demonstrated through acase study on teaching phlebotomy. The case study illustrates how the framework supports the systematic integration ofSSGs into educational curricula. Specifically, the approach enables the comparison, selection, and alignment of SSGs withstudent learning objectives, ensuring their effective use in achieving desired educational outcomes. By providing a structuredapproach to evaluating and integrating SSGs, the TIE-SSG framework facilitates evidence-based decision-making ineducational contexts. This systematic method helps educators align SSGs with learning goals, enhancing the overalleffectiveness of their use in curricula. The framework offers a robust tool for addressing the challenges of incorporating SSGsinto diverse learning pathways.

• Frøland, Tord Waage; Heldal, Ilona & Ersvær, Elisabeth (2025). Towards a Tasks-Interactions-Environment (TIE-SSG) Framework Guiding Integration of Serious Games in Education for Enhanced Practical, Clinical Competences. Proceedings of the European Conference on Games Based Learning (ECGBL). ISSN 2049-0992. 19(2), s. 920–928. doi: 10.34190/ecgbl.19.2.4220. Fulltekst i vitenarkiv Vis sammendrag

  Clinical skills training is essential but resource-intensive for both educational programs and clinical placementsettings. While simulations and serious games (SSGs) show great potential in alleviating skill-training challenges by enablingflexible, interactive, and engaging learning situations in safe environments, difficulties remain in moving beyond their basicimplementation and use. The aim of this study is to explore and conceptualize the key elements that form the basis for theproposed Tasks-Interactions-Environment of Simulations and Serious Games (TIE-SSG) Framework. This work aims to initiatediscussions and generate ideas that can inform future efforts in developing and refining the proposed framework. The TIESSGframework is grounded in instructional scaffolding, and through consideration of these theories and an examination ofprevious literature, the fidelity (i.e., how closely a scenario or tool mirrors real-world conditions) of the SSGs was chosen asthe key factor for the framework. The fidelity term is examined and redefined specifically for introducing SSGs in the contextof teaching and learning, and is broken down into three dimensions: tasks, interactions, and environment. The paperillustrates the benefits of examining the fidelity of these dimensions separately, to help place SSGs within the students’learning pathway in a progressive manner. This approach enhances the understanding of how fidelity impacts learningoutcomes and provides valuable insights for designing practical learning tools. Future research will further explore how thisframework can be applied across diverse educational contexts.

• Bjørkevoll, Sol Maja Graasvold; O'Keeffe, Maria; Konijnenberg, Carolien; Solvik, Beate Stokke; Sødal, Alida & Kaldenbach, Siri [Vis alle 13 forfattere av denne artikkelen] (2025). Infant Vitamin B12 Status and its Predictors – Cross-Sectional Baseline Results from an Ongoing Randomized Controlled Trial. American Journal of Clinical Nutrition. ISSN 0002-9165. 122(3), s. 803–810. doi: 10.1016/j.ajcnut.2025.06.029. Fulltekst i vitenarkiv Vis sammendrag

  Background: Vitamin B12 is a crucial micronutrient for infant growth and development. Objective: The objective of this study was to describe vitamin B12 status in Norwegian infants aged 6–15 wk using multiple biomarkers and cut-off approaches, and to identify its predictors. Methods: From November 2021 through August 2024, infants aged 6–15 wk and their mothers were recruited from public health clinics in Innlandet County, Norway, as part of an ongoing randomized controlled trial. Plasma cobalamin and methylmalonic acid (MMA) concentrations were analyzed among all infants in the cohort (n = 644), and total homocysteine (tHcy) concentrations were analyzed in a subgroup (n = 358). The combined indicator for vitamin B12 status (cB12) was calculated by Fedosov’s equation. Low status was defined using multiple cut-off approaches. Potential predictors of infant vitamin B12 status were evaluated using regression models. Results: Mean (standard deviation [SD]) infant age was 9.1 (1.8) wk. The median (interquartile range) concentrations were: cobalamin 242 (192, 322) pmol/L, tHcy 7.4 (6.2, 9.4) μmol/L, and MMA 0.34 (0.21, 0.77) μmol/L. The mean (SD) cB12 was −0.5 (0.7). Eight percent had cobalamin <148 pmol/L, and 40% <221 pmol/L. Sixty-seven percent had tHcy >6.5 μmol/L, 19% >10 μmol/L, and 4% >13 μmol/L. Sixty-four percent had MMA>0.26 μmol/L. Exclusively breastfed infants had 40% lower cobalamin and 30% higher tHcy compared with nonbreastfed infants. Partially breastfed infants had 21% lower cobalamin and 12% higher tHcy compared with nonbreastfed infants. Conclusion: A substantial proportion of Norwegian infants have biochemical signs of low vitamin B12 status, given that the cut-offs were established in adults. Lower status was observed in partially and exclusively breastfed infants, compared with nonbreastfed infants. However, it is unclear whether these biomarker patterns have clinical significance. Further research is needed to determine consequences of low vitamin B12 biomarker concentrations in early infancy.

• Ryningen, Anita; Rostad, Kari; Ersvær, Elisabeth; Sjøholt, Gry; Paulsen, Gøran & Gundersen, Hilde [Vis alle 8 forfattere av denne artikkelen] (2024). Acute response in circulating microRNAs following a single bout of short-sprint and heavy strength training in well-trained cyclists. Frontiers in Physiology. 15. doi: 10.3389/fphys.2024.1365357. Fulltekst i vitenarkiv Vis sammendrag

  Background: Heavy strength (HS) and short-sprint (SS) are commonly used training methods for competitive road cyclists, with the aim to improve the anaerobic power and short time cycling performance. Knowledge of how such training methods affects biochemical as well as molecular factors, are particularly important for determining individual recovery and long-term adaptations. The primary aim of the current study was to investigate the expression levels of small non-coding RNAs in response to HS and SS training in elite cyclists as potential biomarkers for individual optimal restitution time. Methods: Eleven well trained cyclists performed one session of HS training and one session of SS training on separate days. Blood samples were taken at baseline and 5 min, 1 h and 21 h post training. Along with physiological measurements and biochemical factors (serum creatine kinase, myoglobin, human growth hormone and plasma lactate), real-time quantitative PCR was used to explore whether HS and/or SS training influenced the abundance of 24 circulating miRNAs, in serum, associated with muscle development, angiogenesis, and/or inflammation. Results: Based on complete miRNA profiles from nine cyclists, the miRNAs showing most altered expression after both training sessions included the three striated muscle-specific miRNAs (myomiRs) miR-1-3p, 133a-3p and 133b-3p. While all three miRNAs showed significantly highest expression at 1 h post HS session, the acute effect of the SS session included a significantly higher level of miR-1-3p alone, at 5 min (highest), as well as at 1 h and 21 h post session. Correlation (negative) with biochemical markers was only shown for miR-133a-3p and CK (r = −0.786, p = 0.041) and between miR-133b-3p and [La−] (r = −0.711, p = .032), at 21 h post SS session. Conclusion: Our findings support that unique myomiRs are regulated by HS and SS training. Such knowledge may be important for individually adjusted restitution times.

• Ersvær, Elisabeth; Breistein, Rebecca Irene; Mjøen, Andreas Fossum; Stenseth, Else-Berit & Lid, Olgunn Sivertsen (2022). Praksisveiledning: Behov, ønsker og forventninger. Bioingeniøren. ISSN 0801-6828. 57(1), s. 24–29. Fulltekst i vitenarkiv Vis sammendrag

  Hensikt: Ekstern praksis er en viktig del av en bioingeniørutdanning. I praksisstudier møter bioingeniørstudenten erfarne bioingeniører som sine praksisveiledere. Hensikten med denne studien er å beskrive behov og ønsker praksisveiledere innenfor bioingeniørfag har, for å fremme praksisstudier av høy kvalitet. Metode: «Skulle ønske jeg hadde»-seminar etterfulgt av semistrukturerte dybdeintervjuer ble gjennomført med bioingeniører som er praksisveiledere. En kvalitativ innholdsanalyse ble gjennomført hvor mønstre i datamaterialet ble identifisert og kategorisert. Resultat: Våre funn indikerer at praksisveiledere har ulike behov, ønsker og innspill som kan kategoriseres til «Før praksisperioden», «I praksisperioden», «Etter praksisperioden» og generelle rammebetingelser. Praksisveilederne trenger avsatt tid til både å forberede seg og til å gjennomføre veiledning, og behov/ønsker som identifiseres inkluderer (i) kompetanseheving innenfor veiledning, (ii) fora for erfaringsutveksling, (iii) praksisveiledning i team, (iii) mer kontakt, dialog og forventningsavklaringer med utdanningen (og studenten), (iv) harmoniserte praksissteder og (v) tilbakemeldinger om og evaluering av praksisperioden. Konklusjon: Praksisveiledere innenfor bioingeniørfag har behov, ønsker, utfordringer og forventninger som bør kartlegges – med hensikt å fremme kvalitet i utdanningene.

• Frøland, Tord Hettervik; Heldal, Ilona; Sjøholt, Gry & Ersvær, Elisabeth (2022). Exploring 360°-videos as Realistic Game-based Learning Environments for Work Placement Preparation. NIKT: Norsk IKT-konferanse for forskning og utdanning. ISSN 1892-0713. 1(1). Fulltekst i vitenarkiv Vis sammendrag

  Serious games in immersive virtual reality (VR) environments promise enhanced experiences and engagement, supporting training and learning. While realistically replicating the visuals of real environments can be both difficult and time-consuming, there are many potential advantages from doing so. In real work placements, students enter facilities with variations in types of equipment, rules for navigation, and workflows, adding cognitive load to a usually stressful situation. Allowing them to train and learn in more realistic environments corresponding to their future work placements, with high-fidelity representations, can be time-saving, support core task focus, and help students become more isolated from distractions. This paper presents the VR game 360Phlebotomy, which combines virtual tasks with 360° images and -videos of real laboratories in immersive VR. The game is aimed at helping students become familiar with real workplaces while also learning their work tasks. Current scenarios are tailored to biomedical laboratory science (BLS) education, with a focus on the phlebotomy process, which also serves as the main case in this paper. New environments can be added through a modular scene creation system, with the goal of allowing students to prepare for different work placements. Insights are provided by presenting the design and development process for a prototype tested by five BLS teachers and six BLS students. The results contribute to an increased understanding of the role of context in training and learning and provide a foundation for future work.

• Furuseth, May-Tove; Kvissel, Anne Katrine; Berg, Vivian; Kjøbli, Eli; Volden, Vivi & Solli, Ann Iren [Vis alle 12 forfattere av denne artikkelen] (2022). Praksisstudienes omfang og organisering i bioingeniørutdanningen. Bioingeniøren. ISSN 0801-6828. 8(8), s. 24–29. Fulltekst i vitenarkiv Vis sammendrag

  Siden 2005 har omfang og innhold i bioingeniørutdanningen vært definert av en nasjonal rammeplan for å sikre likeverdig sluttkompetanse for alle kandidater. Fra 2019 ble en ny forskrift om nasjonale retningslinjer fastsatt. Både tidligere rammeplan og nye retningslinjer har regulert læringsutbyttene og omfang av praksis i utdanningen. I denne studien kartlegges omfang og organisering av praksisstudiene ved de ulike studieprogrammene, dette for å undersøke forskjeller og likheter og tilrettelegge for at studiestedene kan lære av hverandre. Det er innhentet data fra hvert studieprogram for omfang og organisering av veiledet intern praksis, ekstern praksis samt bacheloroppgaven. Dette ble kartlagt for alle studiestedene før og etter innføring av nye retningslinjer. Resultatene viser ulikheter i omfang og organisering av praksisstudiene og bacheloroppgaven mellom studieprogrammene i Norge og da særlig i organisering av intern praksis. De nye retningslinjene åpner samtidig opp for en videre fortolkning av praksisbegrepet og av krav til omfang og organisering av praksis. For å sikre likeverdig sluttkompetanse for ferdigutdannede, uavhengig av studiested, er det behov for i større grad enes om hva som gir tilstrekkelig omfang og god kvalitet i praksis i bioingeniørutdanningen. Denne deskriptive studien viser behov for videre forskningssamarbeid mellom studiestedene, for å utvikle en bioingeniørutdanning av best mulig kvalitet for fremtiden.

• Tvedt, Tor Henrik Anderson; Rose-John, Stefan; Tsykunova, Galina; Ahmed, Aymen B; Gedde-Dahl, Tobias & Ersvær, Elisabeth [Vis alle 7 forfattere av denne artikkelen] (2022). IL-6 Responsiveness of CD4⁺ and CD8⁺ T Cells after Allogeneic Stem Cell Transplantation Differs between Patients and Is Associated with Previous Acute Graft versus Host Disease and Pretransplant Antithymocyte Globulin Therapy. Journal of Clinical Medicine. 11(9). doi: 10.3390/jcm11092530. Fulltekst i vitenarkiv Vis sammendrag

  Graft-versus-host disease (GVHD), one of the most common and serious complications after allogeneic stem cell transplantation, is mediated by allocative T cells. IL-6 mediates both pro- and anti-inflammatory effects and modulates T cell response through classical signaling and trans-signaling. We investigated the effects on the mTOR and JAK/STAT pathways after various types of IL-6 signaling for circulating T cells were derived from 31 allotransplant recipients 90 days post-transplant. Cells were stimulated with IL-6 alone, hyper-IL-6 (trans-signaling), IL-6+IL-6 receptor (IL-6R; classical + trans-signaling) and IL-6+IL-6R+soluble gp130-Fc (classical signaling), and flow cytometry was used to investigate the effects on phosphorylation of AKT (Thr308), mTOR (Ser2442), STAT3 (Ser727) and STAT3 (Tyr705). CD3+CD4+ and CD3+C8+ T cells responded to classical and trans IL-6 stimulation with increased STAT3 (Tyr705) phosphorylation; these responses were generally stronger for CD3+CD4+ cells. STAT3 (Tyr705) responses were stronger for patients with previous acute GVHD; CD3+CD4+ cells from GVHD patients showed an additional STAT3 (Ser727) response, whereas patients without acute GVHD showed additional mTOR (Ser2448) responses. Furthermore, treatment with antithymocyte globulin as a part of GVHD prophylaxis was associated with generally weaker STAT3 (Tyr705) responses and altered STAT3 (Ser727) responsiveness of CD3+CD4+ cells together with increased mTOR (Ser2448) responses for the CD3+CD8+ cells. Thus, early post-transplant CD3+CD4+ and CD3+ CD8+ T cell subsets differ in their IL-6 responsiveness; this responsiveness is modulated by antithymocyte globulin and differs between patients with and without previous acute GVHD. These observations suggest that allotransplant recipients will be heterogeneous with regard to the effects of post-transplant IL-6 targeting.

• Frøland, Tord Hettervik; Heldal, Ilona; Braseth, Turid Aarhus; Nygård, Irene; Sjøholt, Gry & Ersvær, Elisabeth (2022). Digital Game-Based Support for Learning the Phlebotomy Procedure in the Biomedical Laboratory Scientist Education. Computers. 11(5). doi: 10.3390/computers11050059. Fulltekst i vitenarkiv Vis sammendrag

  Practice-based training in education is important, expensive, and resource-demanding. Digital games can provide complementary training opportunities for practicing procedural skills and increase the value of the limited laboratory training time in biomedical laboratory science (BLS) education. This paper presents how a serious game can be integrated in a BLS course and supplement traditional learning and teaching with accessible learning material for phlebotomy. To gather information on challenges relevant to integrating Digital Game-Based Learning (DGBL), a case was carried out using mixed methods. Through a semester-long study, following a longitudinal, interventional cohort study, data and information were obtained from teachers and students about the learning impact of the current application. The game motivated students to train more, and teachers were positive towards using it in education. The results provide increased insights into how DGBL can be integrated into education and give rise to a discussion of the current challenges of DGBL for practice-based learning.

• Dasa, Marcus Småvik; Kristoffersen, Morten; Ersvær, Elisabeth; Bovim, Lars Peder Vatshelle; Bjørkhaug, Lise & Moe-Nilssen, Rolf [Vis alle 8 forfattere av denne artikkelen] (2021). The Female Menstrual Cycles Effect on Strength and Power Parameters in High-Level Female Team Athletes. Frontiers in Physiology. 12. doi: 10.3389/fphys.2021.600668. Fulltekst i vitenarkiv Vis sammendrag

  Purpose: The female menstrual cycle (MC) is characterized by hormonal fluctuations throughout its different phases. However, research regarding its effect on athletic performance in high level athletes is sparse. The aim of this study was to (i) investigate the female MCs effect on strength and power performance in highly trained female team athletes throughout the MC and (ii) examine whether eumenorrheic participants with natural hormonal fluctuations displayed enhanced performance in the follicular phase (FP) versus the luteal phase (LP), compared to controls using hormonal contraceptives. Materials and Methods: A total of 29 athletes (Age 21.2 ± 3.3 years; weight 65.6 ± 8.7 kg; height 170.2 ± 8.0 cm; and fat free mass 52.7 ± 7.1) completed the study after a 6-week testing period (8 eumenorrheic participants and 21 hormonal contraceptive controls). Participants were recruited from the team sports soccer, handball and volleyball. Testing protocol consisted of maximal voluntary isometric grip strength, 20-m sprint, countermovement jump and pneumatic leg-press. Based on self-reported use of hormonal contraceptives, participants were divided into non-hormonal contraceptive group and hormonal contraceptive group, the latter working as a control group. Differences in performance between the FP and LP were investigated. MC phase was confirmed by serum hormonal levels through venous blood samples in the non-hormonal contraceptive group. Results: There were no statistically significant changes for the two different phases of the MC, in terms of physical performance for the whole group. Further, there was no significant difference between groups during the MC for any of the outcome variables, maximal voluntary isometric grip strength F(3.29) = 0.362; 20-m sprint F(3.24) = 0.710; countermovement jump F(3.26) = 2.361; and leg-press F(3.26) = 1.746. Conclusion: In high level female team athletes, no difference in performance was observed based on hormonal contraceptive status. This suggests that the MC does not alter acute strength and power performance on a group level in high level team athletes.

• Rundgren, Ida Marie; Ersvær, Elisabeth; Ahmed, Aymen B; Ryningen, Anita & Bruserud, Øystein (2020). A pilot study of circulating monocyte subsets in patients treated with stem cell transplantation for high-risk hematological malignancies. Medicina. ISSN 1010-660X. 56(1), s. 1–14. doi: 10.3390/medicina56010036. Fulltekst i vitenarkiv Vis sammendrag

  Background and Objectives: Autologous and allogeneic stem cell transplantation is used in the treatment of high-risk hematological malignancies, and monocytes are probably involved in hematological reconstitution as well as posttransplant immunoregulation. The aim of our study was to investigate the levels of circulating monocyte subsets in allotransplant recipients. Materials and Methods: The levels of the classical, intermediate, and nonclassical monocyte subsets were determined by flow cytometry. Sixteen patients and 18 healthy controls were included, and the levels were analyzed during pretransplant remission (n = 13), early posttransplant during cytopenia (n = 9), and early reconstitution (n = 9). Results: Most patients in remission showed a majority of classical monocytes. The patients showed severe early posttransplant monocytopenia, but the total peripheral blood monocyte counts normalized very early on, and before neutrophil and platelet counts. During the first 7–10 days posttransplant (i.e., during cytopenia) a majority of the circulating monocytes showed a nonclassical phenotype, but later (i.e., 12–28 days posttransplant) the majority showed a classical phenotype. However, the variation range of classical monocytes was wider for patients in remission and during regeneration than for healthy controls. Conclusions: The total peripheral blood monocyte levels normalize at the very early stages and before neutrophil reconstitution after stem cell transplantation, and a dominance of classical monocytes is reached within 2–4 weeks posttransplant.

• Rundgren, Ida Marie; Ryningen, Anita; Tvedt, Tor Henrik Anderson; Bruserud, Øystein & Ersvær, Elisabeth (2020). Immunomodulatory drugs alter the metabolism and the extracellular release of soluble mediators by normal monocytes. Molecules. ISSN 1431-5157. 25:367(2), s. 1–19. doi: 10.3390/molecules25020367. Fulltekst i vitenarkiv Vis sammendrag

  Immunomodulatory drugs (IMiDs) are used in the treatment of hematological malignancies, especially multiple myeloma. IMiDs have direct anticancer effects but also indirect effects via cancer-supporting stromal cells. Monocytes are a stromal cell subset whose metabolism is modulated by the microenvironment, and they communicate with neighboring cells through extracellular release of soluble mediators. Toll-like receptor 4 (TLR4) is then a common regulator of monocyte metabolism and mediator release. Our aim was to investigate IMiD effects on these two monocyte functions. We compared effects of thalidomide, lenalidomide, and pomalidomide on in vitro cultured normal monocytes. Cells were cultured in medium alone or activated by lipopolysaccharide (LPS), a TLR4 agonist. Metabolism was analyzed by the Seahorse XF 96 cell analyzer. Mediator release was measured as culture supernatant levels. TLR4 was a regulator of both monocyte metabolism and mediator release. All three IMiDs altered monocyte metabolism especially when cells were cultured with LPS; this effect was strongest for lenalidomide that increased glycolysis. Monocytes showed a broad soluble mediator release profile. IMiDs decreased TLR4-induced mediator release; this effect was stronger for pomalidomide than for lenalidomide and especially thalidomide. To conclude, IMiDs can alter the metabolism and cell–cell communication of normal monocytes, and despite their common molecular target these effects differ among various IMiDs.

• Cassetta, Luca; Bruderek, Kirsten; Skrzeczynska-Moncznik, Joanna; Osiecka, Oktawia; Hu, Xiaoying & Rundgren, Ida Marie [Vis alle 44 forfattere av denne artikkelen] (2020). Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation. Journal for ImmunoTherapy of Cancer (JITC). 8:e001223(2), s. 1–13. doi: 10.1136/jitc-2020-001223. Fulltekst i vitenarkiv Vis sammendrag

  Background Myeloid-derived suppressor cells (MDSC) are a functional myeloid cell subset that includes myeloid cells with immune suppressive properties. The presence of MDSC has been reported in the peripheral blood of patients with several malignant and non-malignant diseases. So far, direct comparison of MDSC across different diseases and Centers is hindered by technical pitfalls and a lack of standardized methodology. To overcome this issue, we formed a network through the COST Action Mye-EUNITER (www.mye-euniter.eu) with the goal to standardize and facilitate the comparative analysis of human circulating MDSC in cancer, inflammation and infection. In this manuscript, we present the results of the multicenter study Mye-EUNITER MDSC Monitoring Initiative, that involved 13 laboratories and compared circulating MDSC subsets across multiple diseases, using a common protocol for the isolation, identification and characterization of these cells. Methods We developed, tested, executed and optimized a standard operating procedure for the isolation and immunophenotyping of MDSC using blood from healthy donors. We applied this procedure to the blood of almost 400 patients and controls with different solid tumors and non-malignant diseases. The latter included viral infections such as HIV and hepatitis B virus, but also psoriasis and cardiovascular disorders. Results We observed that the frequency of MDSC in healthy donors varied substantially between centers and was influenced by technical aspects such as the anticoagulant and separation method used. Expansion of polymorphonuclear (PMN)-MDSC exceeded the expansion of monocytic MDSC (M-MDSC) in five out of six solid tumors. PMN-MDSC expansion was more pronounced in cancer compared with infection and inflammation. Programmed death-ligand 1 was primarily expressed in M-MDSC and e-MDSC and was not upregulated as a consequence of disease. LOX-1 expression was confined to PMN-MDSC. Conclusions This study provides improved technical protocols and workflows for the multi-center analysis of circulating human MDSC subsets. Application of these workflows revealed a predominant expansion of PMN-MDSC in solid tumors that exceeds expansion in chronic infection and inflammation.

• Frøland, Tord Hettervik; Heldal, Ilona; Sjøholt, Gry & Ersvær, Elisabeth (2020). Games on mobiles via web or virtual reality technologies: How to support learning for biomedical laboratory science education. Information. 11:195(4), s. 1–16. doi: 10.3390/info11040195. Fulltekst i vitenarkiv Vis sammendrag

  Simulations, serious games, and virtual reality (SSG) applications represent promising support for achieving practical proficiency, but it is difficult to know how to introduce them into a new environment. This paper aims to contribute to a better understanding of introducing new SSGs to a non-computer related educational environment—biomedical laboratory science (BLS) education. By following the choice, construction, and evaluation of a gamified app for practicing phlebotomy (StikkApp), not only the usefulness of the application, but also the general needs and possibilities for supporting SSG applications, are discussed. This paper presents the evaluation of StikkApp through an experimental study examining its use on mobile devices, as a web app and by discussing challenges for a corresponding virtual reality app by BLS students and their teachers. This evaluation focused on questions concerning usage scenarios, technologies, and how the design of the app can be aligned to learning goals necessary for education. By discussing these requirements and possibilities for apps and technology support for using SSG apps for BLS students, this paper contributes to a better understanding of using digital support for sustainable education.

• Rundgren, Ida Marie; Ersvær, Elisabeth; Ahmed, Aymen B; Ryningen, Anita & Bruserud, Øystein (2019). Circulating monocyte subsets in multiple myeloma patients receiving autologous stem cell transplantation – a study of the preconditioning status and the course until posttransplant reconstitution for a consecutive group of patients. BMC Immunology. 20(1), s. 1–13. doi: 10.1186/s12865-019-0323-y. Fulltekst i vitenarkiv Vis sammendrag

  Background Induction therapy of multiple myeloma patients prior to autologous stem cell transplantation has changed from conventional chemotherapy to treatment based on proteasome inhibitors or immunomodulatory drugs. We used flow cytometry to analyze total monocyte and monocyte subset (classical, intermediate and non-classical monocytes) peripheral blood levels before and following auto-transplantation for a consecutive group of myeloma patients who had received the presently used induction therapy. Results The patients showed normal total monocyte concentrations after induction/stem cell mobilization, but the concentrations of classical monocytes were increased compared with healthy controls. Melphalan conditioning reduced the levels of total CD14+ as well as classical and non-classical monocytes, whereas intermediate monocytes were not affected. Thus, melphalan has a non-random effect on monocyte subsets. Melphalan had a stronger effect on total and classical monocyte concentrations for those patients who had received induction therapy including immunomodulatory drugs. Total monocytes and monocyte subset concentrations decreased during the period of pancytopenia, but monocyte reconstitution occurred before hematopoietic reconstitution. However, the fractions of various monocyte subsets varied considerably between patients. Conclusions The total level of circulating monocytes is normalized early after auto-transplantation for multiple myeloma, but pre- and post-transplant levels of various monocyte subsets show considerable variation between patients.

• Bizymi, Nikoleta; Bjelica, Sunčica; Kittang, Astrid Marta Olsnes; Mojsilovic, Slavko; Velegraki, Maria & Pontikoglou, Charalampos [Vis alle 11 forfattere av denne artikkelen] (2019). Myeloid-Derived Suppressor Cells in Hematologic Diseases: Promising Biomarkers and Treatment Targets. HemaSphere. 3:e168(1), s. 1–12. doi: 10.1097/HS9.0000000000000168. Fulltekst i vitenarkiv
• Rundgren, Ida Marie; Bruserud, Øystein; Ryningen, Anita & Ersvær, Elisabeth (2018). Standardization of sampling and sample preparation for analysis of human monocyte subsets in peripheral blood. Journal of Immunological Methods (JIM). ISSN 0022-1759. 461, s. 53–62. doi: 10.1016/j.jim.2018.06.003. Fulltekst i vitenarkiv
• Melve, Guro Kristin; Ersvær, Elisabeth; Eide, Geir Egil; Kristoffersen, Einar Klæboe & Bruserud, Øystein (2018). Peripheral blood stem cell mobilization in healthy Donors by granulocyte colony-stimulating factor causes preferential mobilization of lymphocyte subsets. Frontiers in Immunology. 9:845(MAY), s. 1–15. doi: 10.3389/fimmu.2018.00845. Fulltekst i vitenarkiv Vis sammendrag

  Background: Allogeneic hematopoietic stem cell transplantation is associated with a high risk of immune-mediated post-transplant complications. Graft depletion of immunocompetent cell subsets is regarded as a possible strategy to reduce this risk without reducing antileukemic immune reactivity. Study design and methods: We investigated the effect of hematopoietic stem cell mobilization with granulocyte colony-stimulating factor (G-CSF) on peripheral blood and stem cell graft levels of various T, B, and NK cell subsets in healthy donors. The results from flow cytometric cell quantification were examined by bioinformatics analyses. Results: The G-CSF-induced mobilization of lymphocytes was a non-random process with preferential mobilization of naïve CD4+ and CD8+ T cells together with T cell receptor αβ+ T cells, naïve T regulatory cells, type 1 T regulatory cells, mature and memory B cells, and cytokine-producing NK cells. Analysis of circulating lymphoid cell capacity to release various cytokines (IFNγ, IL10, TGFβ, IL4, IL9, IL17, and IL22) showed preferential mobilization of IL10 releasing CD4+ T cells and CD3−19− cells. During G-CSF treatment, the healthy donors formed two subsets with generally strong and weaker mobilization of immunocompetent cells, respectively; hence the donors differed in their G-CSF responsiveness with regard to mobilization of immunocompetent cells. The different responsiveness was not reflected in the graft levels of various immunocompetent cell subsets. Furthermore, differences in donor G-CSF responsiveness were associated with time until platelet engraftment. Finally, strong G-CSF-induced mobilization of various T cell subsets seemed to increase the risk of recipient acute graft versus host disease, and this was independent of the graft T cell levels. Conclusion: Healthy donors differ in their G-CSF responsiveness and preferential mobilization of immunocompetent cells. This difference seems to influence post-transplant recipient outcomes.

• Kristoffersen, Morten; Sandbakk, Øyvind; Tønnessen, Espen; Svendsen, Ida S.; Paulsen, Gøran & Ersvær, Elisabeth [Vis alle 13 forfattere av denne artikkelen] (2018). Power production and biochemical markers of metabolic stress and muscle damage following a single bout of short-sprint and heavy strength exercise in well-trained cyclists. Frontiers in Physiology. 9:155, s. 1–9. doi: 10.3389/fphys.2018.00155. Fulltekst i vitenarkiv Vis sammendrag

  Purpose: Although strength and sprint training are widely used methods in competitive cycling, no previous studies have compared the acute responses and recovery rates following such sessions among highly trained cyclists. The primary aim of the current study was to compare power production and biochemical markers of metabolic stress and muscle damage following a session of heavy strength (HS) and short-sprint training (SS). Methods: Eleven well-trained male cyclists (18 ± 2 years with maximal oxygen uptake of 67.2 ± 5.0 mL·kg−1·min−1) completed one HS session and one SS session in a randomized order, separated by 48 h. Power production and biochemical variables were measured at baseline and at different time points during the first 45 h post exercise. Results: Lactate and human growth hormone were higher 5 min, 30 min and 1 h post the SS compared to the HS session (all p ≤ 0.019). Myoglobin was higher following the HS than the SS session 5 min, 30 min and 1 h post exercise (all p ≤ 0.005), while creatine kinase (CK) was higher following the HS session 21 and 45 h post exercise (p ≤ 0.038). Counter movement jump and power production during 4 sec sprint returned to baseline levels at 23 and 47 h with no difference between the HS and SS session, whereas the delayed muscle soreness score was higher 45 h following the HS compared to the SS session (p = 0.010). Conclusion: Our findings indicate that SS training provides greater metabolic stress than HS training, whereas HS training leads to more muscle damage compared to that caused by SS training. The ability to produce power remained back to baseline already 23 h after both training sessions, indicating maintained performance levels although higher CK level and muscle soreness were present 45 h post the HS training session.

• Melve, Guro Kristin; Ersvær, Elisabeth; Rye, Kristin Paulsen; Ahmed, Aymen B; Kristoffersen, Einar Klæboe & Hervig, Tor [Vis alle 9 forfattere av denne artikkelen] (2018). The healthy donor profile of immunoregulatory soluble mediators is altered by stem cell mobilization and apheresis. Cytotherapy. ISSN 1465-3249. 20(5), s. 740–754. doi: 10.1016/j.jcyt.2018.02.366. Fulltekst i vitenarkiv
• Tvedt, Tor Henrik Anderson; Ersvær, Elisabeth; Tveita, Anders & Bruserud, Øystein (2017). Interleukin-6 in allogeneic stem cell transplantation: Its possible importance for immunoregulation and as a therapeutic target. Frontiers in Immunology. 8:667, s. 1–15. doi: 10.3389/fimmu.2017.00667. Fulltekst i vitenarkiv Vis sammendrag

  Allogeneic stem cell transplantation is associated with a high risk of treatment-related mortality mainly caused by infections and graft-versus-host disease (GVHD). GVHD is characterized by severe immune dysregulation and impaired regeneration of different tissues, i.e., epithelial barriers and the liver. The balance between pro- and anti-inflammatory cytokine influences the risk of GVHD. Interleukin-6 (IL-6) is a cytokine that previously has been associated with pro-inflammatory effects. However, more recent evidence from various autoimmune diseases (e.g., inflammatory bowel disease, rheumatoid arthritis) has shown that the IL-6 activity is more complex with important effects also on tissue homeostasis, regeneration, and metabolism. This review summarizes the current understanding of how pro-inflammatory IL-6 effects exerted during the peritransplant period shapes T-cell polarization with enhancement of Th17 differentiation and suppression of regulatory T cells, and in addition we also review and discuss the results from trials exploring non-selective IL-6 inhibition in prophylaxis and treatment of GVHD. Emerging evidence suggests that the molecular strategy for targeting of IL-6-initiated intracellular signaling is important for the effect on GVHD. It will therefore be important to further characterize the role of IL-6 in the pathogenesis of GVHD to clarify whether combined IL-6 inhibition of both trans- (i.e., binding of the soluble IL-6/IL-6 receptor complex to cell surface gp130) and cis-signaling (i.e., IL-6 ligation of the IL-6 receptor/gp130 complex) or selective inhibition of trans-signaling should be tried in the prophylaxis and/or treatment of GVHD in allotransplant patients.

• Melve, Guro Kristin; Ersvær, Elisabeth; Akkøk, Cigdem Ahaein; Ahmed, Aymen B; Kristoffersen, Einar Klæboe & Hervig, Tor [Vis alle 7 forfattere av denne artikkelen] (2016). Immunomodulation induced by stem cell mobilization and harvesting in healthy donors: Increased systemic osteopontin levels after treatment with granulocyte colony-stimulating factor. International Journal of Molecular Sciences. ISSN 1661-6596. 17:1158(7). doi: 10.3390/ijms17071158. Fulltekst i vitenarkiv Vis sammendrag

  Peripheral blood stem cells from healthy donors mobilized by granulocyte colony-stimulating factor (G-CSF) and harvested by leukapheresis are commonly used for allogeneic stem cell transplantation. The frequency of severe graft versus host disease is similar for patients receiving peripheral blood and bone marrow allografts, even though the blood grafts contain more T cells, indicating mobilization-related immunoregulatory effects. The regulatory phosphoprotein osteopontin was quantified in plasma samples from healthy donors before G-CSF treatment, after four days of treatment immediately before and after leukapheresis, and 18-24 h after apheresis. Myeloma patients received chemotherapy, combined with G-CSF, for stem cell mobilization and plasma samples were prepared immediately before, immediately after, and 18-24 h after leukapheresis. G-CSF treatment of healthy stem cell donors increased plasma osteopontin levels, and a further increase was seen immediately after leukapheresis. The pre-apheresis levels were also increased in myeloma patients compared to healthy individuals. Finally, in vivo G-CSF exposure did not alter T cell expression of osteopontin ligand CD44, and in vitro osteopontin exposure induced only small increases in anti-CD3- and anti-CD28-stimulated T cell proliferation. G-CSF treatment, followed by leukapheresis, can increase systemic osteopontin levels, and this effect may contribute to the immunomodulatory effects of G-CSF treatment.

• Ersvær, Elisabeth; Brenner, Annette; Vetås, Kristin; Reikvam, Håkon & Bruserud, Øystein (2015). Effects of cytarabine on activation of human T cells - cytarabine has concentration-dependent effects that are modulated both by valproic acid and all-trans retinoic acid. BMC Pharmacology and Toxicology. 16:12. doi: 10.1186/s40360-015-0012-2. Fulltekst i vitenarkiv Vis sammendrag

  Background: Cytarabine is used in the treatment of acute myeloid leukemia (AML). Low-dose cytarabine can be combined with valproic acid and all-trans retinoic acid (ATRA) as AML-stabilizing treatment. We have investigated the possible risk of immunotoxicity by this combination. We examined the effects of cytarabine combined with valproic acid and ATRA on in vitro activated human T cells, and we tested cytarabine at concentrations reached during in vivo treatment with high doses, conventional doses and low doses. Methods: T cells derived from blood donors were activated in vitro in cell culture medium alone or supplemented with ATRA (1 μM), valproic acid (500 or 1000 μM) or cytarabine (0.01-44 μM). Cell characteristics were assessed by flow cytometry. Supernatants were analyzed for cytokines by ELISA or Luminex. Effects on primary human AML cell viability and proliferation of low-dose cytarabine (0.01-0.5 μM) were also assessed. Statistical tests include ANOVA and Cluster analyses. Results: Only cytarabine 44 μM had both antiproliferative and proapoptotic effects. Additionally, this concentration increased the CD4:CD8 T cell ratio, prolonged the expression of the CD69 activation marker, inhibited CD95L and heat shock protein (HSP) 90 release, and decreased the release of several cytokines. In contrast, the lowest concentrations (0.35 and 0.01 μM) did not have or showed minor antiproliferative or cytotoxic effects, did not alter activation marker expression (CD38, CD69) or the release of CD95L and HSP90, but inhibited the release of certain T cell cytokines. Even when these lower cytarabine concentrations were combined with ATRA and/or valproic acid there was still no or minor effects on T cell viability. However, these combinations had strong antiproliferative effects, the expression of both CD38 and CD69 was altered and there was a stronger inhibition of the release of FasL, HSP90 as well as several cytokines. Cytarabine (0.01-0.05 μM) showed a dose-dependent antiproliferative effect on AML cells, and in contrast to the T cells this effect reached statistical significance even at 0.01 μM. Conclusions: Even low levels of cytarabine, and especially when combined with ATRA and valproic acid, can decrease T cell viability, alter activation-induced membrane-molecule expression and decrease the cytokine release.

• Reikvam, Håkon; Tamburini, Jerome; Skrede, Silje; Holdhus, Rita; Poulain, Laury & Ersvær, Elisabeth [Vis alle 8 forfattere av denne artikkelen] (2014). Antileukaemic effect of PI3K-mTOR inhibitors in acute myeloid leukaemia-gene expression profiles reveal CDC25B expression as determinate of pharmacological effect. British Journal of Haematology. ISSN 0007-1048. 164(2), s. 200–211. doi: 10.1111/bjh.12611. Fulltekst i vitenarkiv
• Hampson, Peter; Wang, Keqing; Ersvær, Elisabeth; McCormack, Emmet; Schüler, Julia & Fiebig, Heinz-Herbert [Vis alle 9 forfattere av denne artikkelen] (2014). Up-regulation of anti-apoptotic genes confers resistance to the novel anti-leukemic compound PEP005 in primary AML cells. Oncoscience. 1(8), s. 529–539. doi: 10.18632/oncoscience.71. Fulltekst i vitenarkiv
• Reikvam, Håkon; Forthun, Rakel Brendsdal; Brenner, Annette; Ersvær, Elisabeth; Hatfield, Kimberley Joanne & Bruserud, Øystein (2014). Heat shock protein 90 – a potential target in the treatment of human acute myelogenous leukemia. Advances in cancer drug targets. ISSN 2213-9915. 2, s. 3–53. doi: 10.2174/9781608059386114020003. Fulltekst i vitenarkiv
• Bredholt, Therese; Ersvær, Elisabeth; Erikstein, Bjarte Skoe; Sulen, Andre; Reikvam, Håkon & Aarstad, Hans Jørgen [Vis alle 10 forfattere av denne artikkelen] (2013). Distinct single cell signal transduction signatures in leukocyte subsets stimulated with khat extract, amphetamine-like cathinone, cathine or norephedrine. BMC Pharmacology and Toxicology. 14. doi: 10.1186/2050-6511-14-35. Fulltekst i vitenarkiv Vis sammendrag

  Background: Amphetamine and amphetamine derivatives are suggested to induce an immunosuppressive effect. However, knowledge of how amphetamines modulate intracellular signaling pathways in cells of the immune system is limited. We have studied phosphorylation of signal transduction proteins (Akt, CREB, ERK1/2, NF-κB, c-Cbl, STAT1/3/5/6) and stress sensors (p38 MAPK, p53) in human leukocyte subsets following in vitro treatment with the natural amphetamine cathinone, the cathinone derivatives cathine and norephedrine, in comparison with a defined extract of the psychostimulating herb khat (Catha edulis Forsk.). Intracellular protein modifications in single cells were studied using immunostaining and flow cytometry, cell viability was determined by Annexin V-FITC/Propidium Iodide staining, and T-lymphocyte proliferation was measured by 3H-thymidine incorporation. Results: Cathinone, cathine and norephedrine generally reduced post-translational modifications of intracellular signal transducers in T-lymphocytes, B-lymphocytes, natural killer cells and monocytes, most prominently affecting c-Cbl (pTyr700), ERK1/2 (p-Thr202/p-Tyr204), p38 MAPK (p-Thr180/p-Tyr182) and p53 (both total p53 protein and p- Ser15). In contrast, the botanical khat-extract induced protein phosphorylation of STAT1 (p-Tyr701), STAT6 (p- Tyr641), c-Cbl (pTyr700), ERK1/2 (p-Thr202/p-Tyr204), NF-κB (p-Ser529), Akt (p-Ser473), p38 MAPK (p-Thr180/p- Tyr182), p53 (Ser15) as well as total p53 protein. Cathinone, cathine and norephedrine resulted in unique signaling profiles, with B-lymphocytes and natural killer cells more responsive compared to T-lymphocytes and monocytes. Treatment with norephedrine resulted in significantly increased T-lymphocyte proliferation, whereas khat-extract reduced proliferation and induced cell death. Conclusions: Single-cell signal transduction analyses of leukocytes distinctively discriminated between stimulation with cathinone and the structurally similar derivatives cathine and norephedrine. Cathinone, cathine and norephedrine reduced phosphorylation of c-Cbl, ERK1/2, p38 MAPK and p53(Ser15), and norephedrine induced Tlymphocyte proliferation. Khat-extract induced protein phosphorylation of signal transducers, p38 MAPK and p53, followed by reduced cell proliferation and cell death. This study suggests that protein modification-specific singlecell analysis of immune cells could unravel pharmacologic effects of amphetamines and amphetamine-like agents, and further could represent a valuable tool in elucidation of mechanism(s) of action of complex botanical extracts.

• Reikvam, Håkon; Kittang, Astrid Marta Olsnes; Melve, Guro Kristin; Mosevoll, Knut Anders; Bentsen, Pål Tore & Ersvær, Elisabeth [Vis alle 8 forfattere av denne artikkelen] (2013). Targeted anti-leukemic therapy as disease-stabilizing treatment for acute myeloid leukemia relapse after allogeneic stem cell transplantation: will it be possible to combine these strategies with retransplantation or donor lymphocyte infusions? Current Cancer Drug Targets. ISSN 1568-0096. 13(1), s. 30–47. doi: 10.2174/1568009611309010030. Fulltekst i vitenarkiv
• Fredly, Hanne Kristin; Ersvær, Elisabeth; Kittang, Astrid Marta Olsnes; Tsykunova, Galina; Gjertsen, Bjørn Tore & Bruserud, Øystein (2013). The combination of valproic acid, all-trans retinoic acid and low-dose cytarabine as disease-stabilizing treatment in acute myeloid leukemia. Clinical Epigenetics. 5:13. doi: 10.1186/1868-7083-5-13. Fulltekst i vitenarkiv Vis sammendrag

  Background: A large proportion of patients with acute myeloid leukemia (AML) are not fit for intensive and potentially curative therapy due to advanced age or comorbidity. Previous studies have demonstrated that a subset of these patients can benefit from disease-stabilizing therapy based on all-trans retinoic acid (ATRA) and valproic acid. Even though complete hematological remission is only achieved for exceptional patients, a relatively large subset of patients respond to this treatment with stabilization of normal peripheral blood cell counts. Methods: In this clinical study we investigated the efficiency and safety of combining (i) continuous administration of valproic acid with (ii) intermittent oral ATRA treatment (21.5 mg/m2 twice daily) for 14 days and low-dose cytarabine (10 mg/m2 daily) for 10 days administered subcutaneously. If cytarabine could not control hyperleukocytosis it was replaced by hydroxyurea or 6-mercaptopurin to keep the peripheral blood blast count below 50 × 109/L. Results: The study included 36 AML patients (median age 77 years, range 48 to 90 years) unfit for conventional intensive chemotherapy; 11 patients responded to the treatment according to the myelodysplastic syndrome (MDS) response criteria and two of these responders achieved complete hematological remission. The most common response to treatment was increased and stabilized platelet counts. The responder patients had a median survival of 171 days (range 102 to > 574 days) and they could spend most of this time outside hospital, whereas the nonresponders had a median survival of 33 days (range 8 to 149 days). The valproic acid serum levels did not differ between responder and nonresponder patients and the treatment was associated with a decrease in the level of circulating regulatory T cells. Conclusion: Treatment with continuous valproic acid and intermittent ATRA plus low-dose cytarabine has a low frequency of side effects and complete hematological remission is seen for a small minority of patients. However, disease stabilization is seen for a subset of AML patients unfit for conventional intensive chemotherapy.

• Reikvam, Håkon; Hatfield, Kimberley Joanne; Ersvær, Elisabeth; Hovland, Randi; Skavland, Jørn & Gjertsen, Bjørn Tore [Vis alle 8 forfattere av denne artikkelen] (2012). Expression profile of heat shock proteins in acute myeloid leukaemia patients reveals a distinct signature strongly associated with FLT3 mutation status - consequences and potentials for pharmacological intervention. British Journal of Haematology. ISSN 0007-1048. 156(4), s. 468–480. doi: 10.1111/j.1365-2141.2011.08960.x. Fulltekst i vitenarkiv
• Fredly, Hanne Kristin; Ersvær, Elisabeth; Gjertsen, Bjørn Tore & Bruserud, Øystein (2011). Immunogenic apoptosis in human acute myeloid leukemia (AML): primary human AML cells expose calreticulin and release heat shock protein (HSP) 70 and HSP90 during apoptosis. Oncology Reports. ISSN 1021-335X. 25(6), s. 1549–1556. doi: 10.3892/or.2011.1229. Fulltekst i vitenarkiv
• Ersvær, Elisabeth; Melve, Guro Kristin & Bruserud, Øystein (2011). Future perspectives: should Th17 cells be considered as a possible therapeutic target in acute myeloid leukemia patients receiving allogeneic stem cell transplantation? Cancer Immunology and Immunotherapy. ISSN 0340-7004. 60(12), s. 1669–1681. doi: 10.1007/s00262-011-1118-z. Fulltekst i vitenarkiv
• Ersvær, Elisabeth; Hatfield, Kimberley Joanne; Reikvam, Håkon & Bruserud, Øystein (2011). Future perspectives: therapeutic targeting of notch signalling may become a strategy in patients receiving stem cell transplantation for hematologic malignancies. Bone Marrow Research. ISSN 2090-2999. 2011(2011). doi: 10.1155/2011/570796. Fulltekst i vitenarkiv
• Melve, Guro Kristin; Ersvær, Elisabeth; Kittang, Astrid Marta Olsnes & Bruserud, Øystein (2011). The chemokine system. in allogeneic stem-cell transplantation: a possible therapeutic target? Expert Review of Hematology. ISSN 1747-4086. 4(5), s. 563–576. doi: 10.1586/EHM.11.54. Fulltekst i vitenarkiv
• Akkæk, Cigdem Ahaein; Liseth, Knut; Melve, Guro Kristin; Ersvær, Elisabeth; Hervig, Tor & Bruserud, Øystein (2011). Is there a scientific basis for a recommended standardization of collection and cryopreservation of peripheral blood stem cell grafts? Cytotherapy. ISSN 1465-3249. 13(8), s. 1013–1024. doi: 10.3109/14653249.2011.574117. Fulltekst i vitenarkiv
• Liseth, Knut; Ersvær, Elisabeth; Hervig, Tor & Bruserud, Øystein (2010). Combination of Intensive Chemotherapy and Anticancer Vaccines in the Treatment of Human Malignancies: The Hematological Experience. Journal of Biomedicine and Biotechnology. ISSN 1110-7243. doi: 10.1155/2010/692097. Fulltekst i vitenarkiv
• Kisand, Kai; Wolff, Anette Susanne Bøe; Podkrajsek, Katarina Trebusak; Tserel, Liina; Link, Maire & Kisand, Kalle V. [Vis alle 25 forfattere av denne artikkelen] (2010). Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines. Journal of Experimental Medicine (JEM). ISSN 0022-1007. 207(2), s. 299–308. doi: 10.1084/jem.20091669. Fulltekst i vitenarkiv
• Ersvær, Elisabeth; Liseth, Knut; Skavland, Jørn; Gjertsen, Bjørn Tore & Bruserud, Øystein (2010). Intensive chemotherapy for acute myeloid leukemia differentially affects circulating TC1, TH1, TH17 and TREG cells. BMC Immunology. 11. doi: 10.1186/1471-2172-11-38. Fulltekst i vitenarkiv Vis sammendrag

  Background Several observations suggest that immunological events early after chemotherapy, possibly during the period of severe treatment-induced cytopenia, are important for antileukemic immune reactivity in acute myeloid leukemia (AML). We therefore investigated the frequencies of various T cell subsets (TC1, TH1, TH17) and CD25+ FoxP3+ TREG cells in AML patients with untreated disease and following intensive chemotherapy. Results Relative levels of circulating TC1 and TH1 cells were decreased in patients with severe chemotherapy-induced cytopenia, whereas TH17 levels did not differ from healthy controls. Increased levels of regulatory CD25+ FoxP3+ T cells were detected in AML patients with untreated disease, during chemotherapy-induced cytopenia and during regeneration after treatment. TH17 and TH1 levels were significantly higher in healthy males than females, but this gender difference was not detected during chemotherapy-induced cytopenia. Finally, exogenous IL17-A usually had no or only minor effects on proliferation of primary human AML cells. Conclusions We conclude that the effect of intensive AML chemotherapy differ between circulating T cell subsets, relative frequencies of TH17 cells are not affected by chemotherapy and this subset may affect AML cells indirectly through their immunoregulatory effects but probably not through direct effects of IL17-A.

• Hampson, Peter; Wang, Keqing; Milverton, Lisa; Ersvær, Elisabeth; Bruserud, Øystein & Lord, Janet M. (2010). Kinetics of ERK1/2 activation determine sensitivity of acute myeloid leukaemia cells to the induction of apoptosis by the novel small molecule ingenol 3-angelate (PEP005). Apoptosis. ISSN 1360-8185. 15(8), s. 946–955. doi: 10.1007/s10495-010-0507-7. Fulltekst i vitenarkiv
• Wolff, Anette Susanne Bøe; Oftedal, Bergithe Eikeland; Kisand, Kai; Ersvær, Elisabeth; Lima, Kari & Husebye, Eystein Sverre (2010). Flow cytometry study of blood cell subtypes reflects autoimmune and inflammatory processes in autoimmune polyendocrine syndrome type I. Scandinavian Journal of Immunology. ISSN 0300-9475. 71(6), s. 459–467. doi: 10.1111/j.1365-3083.2010.02397.x. Fulltekst i vitenarkiv
• Liseth, Knut; Sjo, Malvin; Paulsen, Kristin; Bruserud, Øystein & Ersvær, Elisabeth (2010). Early pre-engraftment, functional, in vitro responsiveness of T lymphocytes in allotransplanted, acute leukemia patients: proliferation and release of a broad profile of cytokines, possibly predictive of graft-versus-host disease. European Cytokine Network. ISSN 1148-5493. 21(1), s. 40–49. doi: 10.1684/ecn.2009.0181. Fulltekst i vitenarkiv
• Akkæk, Cigdem Ahaein; Hervig, Tor; Bjørsvik, Solfrid; Underdal, Maria Othelie; Skoug, Carl Fredrig & Ersvær, Elisabeth [Vis alle 7 forfattere av denne artikkelen] (2010). Minor diurnal and activity-induced variations in daytime peripheral blood platelet counts do not have any major impact on platelet yield by platelet apheresis. Transfusion and Apheresis Science. ISSN 1473-0502. 43(1), s. 33–36. doi: 10.1016/j.transci.2010.05.005. Fulltekst i vitenarkiv Vis sammendrag

  Skoug, Carl Fredrig: Institutt for indremedisin, ikke i FS./tøf

• Reikvam, Håkon; Hatfield, Kimberley Joanne; Lassalle, Philippe; Kittang, Astrid Marta Olsnes; Ersvær, Elisabeth & Bruserud, Øystein (2010). Targeting the angiopoietin (Ang)/Tie-2 pathway in the crosstalk between acute myeloid leukaemia and endothelial cells: studies of Tie-2 blocking antibodies, exogenous Ang-2 and inhibition of constitutive agonistic Ang-1 release. Expert Opinion on Investigational Drugs. ISSN 1354-3784. 19(2), s. 169–183. doi: 10.1517/13543780903485659. Fulltekst i vitenarkiv
• Olsnes, Astrid Marta; Ersvær, Elisabeth; Ryningen, Anita; Paulsen, Kristin; Hampson, Peter & Lord, Janet M. [Vis alle 9 forfattere av denne artikkelen] (2009). The protein kinase C agonist PEP005 increases NF-kB expression, induces differentiation and increases constitutive chemokine release by primary acute myeloid leukaemia cells. British Journal of Haematology. ISSN 0007-1048. 145(6), s. 761–774. doi: 10.1111/j.1365-2141.2009.07691.x. Fulltekst i vitenarkiv
• Hatfield, Kimberley Joanne; Øyan, Anne Margrete; Ersvær, Elisabeth; Kalland, Karl-Henning; Lassalle, Philippe & Gjertsen, Bjørn Tore [Vis alle 7 forfattere av denne artikkelen] (2009). Phase II study of subcutaneous alemtuzumab without dose escalation in patients with advanced-stage, relapsed chronic lymphocytic leukaemia. British Journal of Haematology. ISSN 0007-1048. 144(1), s. 53–68. doi: 10.1111/j.1365-2141.2008.07411.x. Fulltekst i vitenarkiv
• Reikvam, Håkon; Olsnes, Astrid Marta; Gjertsen, Bjørn Tore; Ersvær, Elisabeth & Bruserud, Øystein (2009). Nuclear Factor-kappaB Signaling: A Contributor in Leukemogenesis and a Target for Pharmacological Intervention in Human Acute Myelogenous Leukemia. Critical reviews in oncogenesis. ISSN 0893-9675. 15(1-2), s. 1–41. Fulltekst i vitenarkiv
• Hatfield, Kimberley Joanne; Øyan, Anne Margrete; Ersvær, Elisabeth; Kalland, Karl-Henning; Lassalle, Philippe & Gjertsen, Bjørn Tore [Vis alle 7 forfattere av denne artikkelen] (2009). Primary human acute myeloid leukaemia cells increase the proliferation of microvascular endothelial cells through the release of soluble mediators. British Journal of Haematology. ISSN 0007-1048. 144(1), s. 53–68. doi: 10.1111/j.1365-2141.2008.07411.x. Fulltekst i vitenarkiv
• Liseth, Knut; Ersvær, Elisabeth; Abrahamsen, Jenny Foss; Nesthus, Ingerid; Ryningen, Anita & Bruserud, Øystein (2009). Long-term cryopreservation of autologous stem cell grafts: a clinical and experimental study of hematopoietic and immunocompetent cells. Transfusion. ISSN 0041-1132. 49(8), s. 1709–1719. doi: 10.1111/j.1537-2995.2009.02180.x. Fulltekst i vitenarkiv
• Reikvam, Håkon; Ersvær, Elisabeth & Bruserud, Øystein (2009). Heat shock protein 90-a potential target in the treatment of human acute myelogenous leukemia. Current Cancer Drug Targets. ISSN 1568-0096. 9(6), s. 761–776. Fulltekst i vitenarkiv
• Akkök, Çigdem Akalin; Liseth, Knut; Hervig, Tor; Ryningen, Anita; Bruserud, Øystein & Ersvær, Elisabeth (2009). Use of different DMSO concentrations for cryopreservation of autologous peripheral blood stem cell grafts does not have any major impact on levels of leukocyte- and platelet-derived soluble mediators. Cytotherapy. ISSN 1465-3249. 11(6), s. 749–760. doi: 10.3109/14653240902980443. Fulltekst i vitenarkiv
• Bruserud, Øystein; Ersvær, Elisabeth; Olsnes, Astrid Marta & Gjertsen, Bjørn Tore (2008). Anticancer Immunotherapy in Combination with Proapoptotic Therapy. Current Cancer Drug Targets. ISSN 1568-0096. 8(8), s. 666–675. doi: 10.2174/156800908786733496. Fulltekst i vitenarkiv
• Olsnes, Astrid Marta; Ersvær, Elisabeth; Ryningen, Anita & Bruserud, Øystein (2008). Circulating T cells derived from acute leukemia patients with severe therapy-induced cytopenia express a wide range of chemokine receptors. Hematology. ISSN 1024-5332. 13(6), s. 329–332. doi: 10.1179/102453308X343491. Fulltekst i vitenarkiv
• Olsnes, Astrid Marta; Ryningen, Anita; Ersvær, Elisabeth & Bruserud, Øystein (2008). In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22. Journal of Interferon and Cytokine Research. ISSN 1079-9907. 28(5), s. 297–310. doi: 10.1089/jir.2007.0052. Fulltekst i vitenarkiv
• Ersvær, Elisabeth; Hampson, Peter; Hatfield, Kimberley Joanne; Ulvestad, Elling; Wendelbo, Øystein & Lord, Janet M. [Vis alle 8 forfattere av denne artikkelen] (2007). T cells remaining after intensive chemotherapy for acute myelogenous leukemia show a broad cytokine release profile including high levels of interferon-gamma that can be further increased by a novel protein kinase C agonist PEP005. Cancer Immunology and Immunotherapy. ISSN 0340-7004. 56(6), s. 013–925. doi: 10.1007/s00262-006-0236-5. Fulltekst i vitenarkiv
• Stapnes, Camilla I; Døskeland, Anne Paulus; Hatfield, Kimberley Joanne; Ersvær, Elisabeth; Ryningen, Anita & Lorens, James [Vis alle 8 forfattere av denne artikkelen] (2007). The proteasome inhibitors bortezomib and PR-171 have antiproliferative and proapoptotic effects on primary human acute myeloid leukaemia cells. British Journal of Haematology. ISSN 0007-1048. 136(6), s. 814–828. doi: 10.1111/j.1365-2141.2007.06504.x. Fulltekst i vitenarkiv
• Ersvær, Elisabeth; Zhang, Jian-Ying; McCormack, Emmet Martin; Olsnes, Astrid Marta; Ånensen, Nina & Tan, Eng M. [Vis alle 8 forfattere av denne artikkelen] (2007). Cyclin B1 is commonly expressed in the cytoplasm of primary human acute myelogenous leukemia cells and serves as a leukemia-associated antigen associated with autoantibody response in a subset of patients. European Journal of Haematology. ISSN 0902-4441. 79(3), s. 210–225. doi: 10.1111/j.1600-0609.2007.00899.x. Fulltekst i vitenarkiv

Se alle arbeider i NVA

• Fossland, Trine; Ersvær, Elisabeth; Hjelsvold, Rune; Østergaard, Edvin; Berg, Arild Skarsfjord & Kubberød, Elin [Vis alle 8 forfattere av denne artikkelen] (2025). Hvordan kan meritterings­ordningen bli en drivkraft for kvalitets­utvikling? doi: https:/www.khrono.no/hvordan-kan-meritteringsordningen-bli-en-drivkraft-for-kvalitetsutvikling/981512. Fulltekst i vitenarkiv Vis sammendrag

  Gjennom nasjonal koordinering og institusjonell forankring kan vi sikre at meritterte undervisere blir en drivkraft for den nødvendige kvalitetsutviklingen, skriver de åtte forfatterne.

• Stenseth, Else Berit; Svarthaug, Lars Aune; Stokke, Randi & Ersvær, Elisabeth (2025). Læring på tvers av profesjoner og geografi – bioingeniørens rolle i et traumeteam. Bioingeniøren. ISSN 0801-6828. s. 22–26. Fulltekst i vitenarkiv Vis sammendrag

  Deltakelse i traumeteam er en liten, men viktig del av bioingeniørens funksjon, og den må læres. Bioingeniørstudenter i Innlandet fikk øve seg i tverrprofesjonelt samarbeid sammen med andre helsefagstudenter i «Traumeuka» ved NTNU i Gjøvik.

• Ersvær, Elisabeth; Lie-Jensen, Anette Christensen; Chrisanthar, Ranjan; Neslein, Inger-Lise F.; Solli, Ann Iren & Alm, Bente [Vis alle 8 forfattere av denne artikkelen] (2025). Bioingeniører må være med å utdanne morgendagens bioingeniører - med hvordan rekrutterer vi dem? s. 26–29. Fulltekst i vitenarkiv
• Ersvær, Elisabeth; Stenseth, Else Berit; Kjøbli, Eli; Møller, Ina; Østby, Runa Berg & Solli, Ann Iren [Vis alle 16 forfattere av denne artikkelen] (2024). CaseMedLab - Caser og digitale læringsressurser innen laboratoriemedisin. Fulltekst i vitenarkiv Vis sammendrag

  Caser og digitale læringsressurser innen laboratoriemedisin

• Kubberød, Elin; Østergaard, Edvin; Berg, Arild; Sørlie, Morten; Hjelsvold, Rune & Ersvær, Elisabeth [Vis alle 8 forfattere av denne artikkelen] (2024). Akademisk staffasje eller pådrivere for pedagogisk utvikling? Fulltekst i vitenarkiv Vis sammendrag

  Meritterte undervisere må gis større muligheter til å være reelle pådrivere for forandring og nødvendig omstilling i sektoren.

• Stenseth, Else Berit; Kvissel, Anne Katrine; Kjøbli, Eli; Lie-Jensen, Anette Christensen; Østby, Runa Berg & Jacobsen, Bente Marie Berntsen [Vis alle 9 forfattere av denne artikkelen] (2023). Bridging the Gap between working life and BLS education programs. Fulltekst i vitenarkiv
• Stenseth, Else Berit; Wergeland, Line; Granerud, Beathe Kiland; Syversen, Linda; Andersen, Heidi & Ersvær, Elisabeth (2023). Conditions for Communication, information and collaboration Between Higher education and healthcare Institutions organising clinical placements: The Biomedical Laboratory Scientists Perspectives. Fulltekst i vitenarkiv
• Ersvær, Elisabeth; Wergeland, Line; Tunsjø, Hege Smith; Neslein, Inger-Lise F.; Lie-Jensen, Anette Christensen & Berg, Vivian [Vis alle 8 forfattere av denne artikkelen] (2023). UHR sier nei! Men vi sier ja! Khrono. Fulltekst i vitenarkiv
• Marienborg, Kaja; Andersen, Heidi & Ersvær, Elisabeth (2023). Innovasjon i helsevesenet - hva venter vi på? Fulltekst i vitenarkiv
• Bakken, Kjersti Sletten; Kvestad, Ingrid; Bjørkevoll, Sol Maja Graasvold; Solvik, Beate Stokke; Kaldenbach, Siri & McCann, Adrian [Vis alle 10 forfattere av denne artikkelen] (2023). Vitamin B12 status in infancy and the effect of a vitamin B12 injection in infants with subclinical vitamin B12 deficiency: study protocol for a register-based randomised controlled trial. BMJ Open. 13(4). doi: 10.1136/bmjopen-2022-069102. Fulltekst i vitenarkiv Vis sammendrag

  Introduction: Vitamin B12 (cobalamin) is crucial for optimal child development and growth, yet deficiency is common worldwide. The aim of this study is twofold; (1) to describe vitamin B12 status and the status of other micronutrients in Norwegian infants, and (2) in a randomised controlled trial (RCT), investigate the effect of vitamin B12 supplementation on neurodevelopment in infants with subclinical vitamin B12 deficiency. Methods and analysis: Infant blood samples, collected at public healthcare clinics, are analysed for plasma cobalamin levels. Infants with plasma cobalamin <148 pmol/L are immediately treated with hydroxocobalamin and excluded from the RCT. Remaining infants (cobalamin ≥148 pmol/L) are randomly assigned (in a 1:1 ratio) to either a screening or a control group. In the screening group, baseline samples are immediately analysed for total homocysteine (tHcy), while in the control group, the baseline samples will be analysed after 12 months. Screening group infants with plasma tHcy >6.5 µmol/L, are given an intramuscular injection of hydroxocobalamin (400 µg). The primary outcomes are cognitive, language and motor development assessed using the Bayley Scales of Infant and Toddler Development at 12 months of age. Ethics and dissemination: The study has been approved by the Regional Committee for Medical and Health Research Ethics (ref: 186505). Investigators who meet the Vancouver requirements will be eligible for authorship and be responsible for dissemination of study findings. Results will extend current knowledge on consequences of subclinical vitamin B12 deficiency during infancy and may inform future infant feeding recommendations. Trial registration number: NCT05005897. Keywords: Developmental neurology & neurodisability; EPIDEMIOLOGY; NUTRITION & DIETETICS. © Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

• Marienborg, Kaja; Ersvær, Elisabeth & Andersen, Heidi (2023). Utdanningene alene kan ikke løse bioingeniør­krisen. Fulltekst i vitenarkiv
• Lie-Jensen, Anette Christensen; Brattelid, Trond; Neslein, Inger-Lise F.; Ersvær, Elisabeth; Alm, Bente & Tunsjø, Hege [Vis alle 8 forfattere av denne artikkelen] (2023). Gir bioingeniørutdanningene gode redskaper for å håndtere og forebygge krenkelser? Fulltekst i vitenarkiv
• Andersen, Heidi; Alm, Bente; Berg, Vivian; Ersvær, Elisabeth; Lie-Jensen, Anette Christensen & Neslein, Inger-Lise F. [Vis alle 9 forfattere av denne artikkelen] (2022). Vil styrke norsk språkkrav for bioingeniører. Fulltekst i vitenarkiv
• Andersen, Heidi; Alm, Bente; Berg, Vivian; Ersvær, Elisabeth; Lie-Jensen, Anette Christensen & Neslein, Inger-Lise F. [Vis alle 8 forfattere av denne artikkelen] (2022). Tenk hva de nasjonale fag­organene innen helse og sosial kunne fått til sammen. Fulltekst i vitenarkiv
• Andersen, Heidi; Alm, Bente; Berg, Vivian; Ersvær, Elisabeth; Lie-Jensen, Anette Christensen & Neslein, Inger-Lise F. [Vis alle 9 forfattere av denne artikkelen] (2022). Vil styrke norsk språkkrav for bio­ingeniører. Fulltekst i vitenarkiv
• Frøland, Tord Hettervik; Heldal, Ilona; Ersvær, Elisabeth & Sjøholt, Gry (2021). Merging 360°-videos and Game-Based Virtual Environments for Phlebotomy Training: Teachers and Students View. Fulltekst i vitenarkiv Vis sammendrag

  This paper presents a Virtual Reality (VR) game aimed at phlebotomy learning in real-life contexts. 360°-images and -videos of existing laboratories were used as the basis of the virtual environment. The game was designed with a focus on problem- and inquiry-based learning through exploration of laboratories with VR mini-games and interaction opportunities for essential procedural learning tasks. The results are based on usability and user experience evaluations from 6 students and 5 educators. Aspects related to games’ attractiveness, engagement and enjoyment were highly rated, though several improvements were deemed necessary before the application should be used in practice. The result contrasts the different opinions of teachers and students. While teachers were more critical regarding usability problems, they were positive towards using gamified experiences for procedural learning. Students wished to have even more gamified tasks and a better flow between tasks or environments.

• Frøland, Tord Hettervik; Heldal, Ilona; Ersvær, Elisabeth & Sjøholt, Gry (2020). State-of-the-art and Future Directions for Using Augmented Reality Head Mounted Displays for First Aid Live Training. Fulltekst i vitenarkiv Vis sammendrag

  Mixed reality and serious games are often advocated as promising technologies in supporting training and increasing the skills necessary to deal with new, complex, and often unexpected situations. This paper explores the feasibility of an Augmented Reality-(AR) based cyber-physical solution to complement live training for the treatment of serious wound injuries after accidents. Based on an experimental study set-up and examining the technology and the involved stakeholders’ experiences, this paper explores current possibilities and limitations for using a Microsoft HoloLens Head Mounted Display (HMD) for first aid trauma training.

• Sjøholt, Gry & Ersvær, Elisabeth (2019). E-LEARNING FACILITATES FLIPPED LEARNING AND PORTFOLIO ASSESSMENT IN BIOMEDICAL LABORATORY SCIENCE. Fulltekst i vitenarkiv
• Sjøholt, Gry; Ryningen, Anita; Bjørkhaug, Lise; Rostad, Kari & Ersvær, Elisabeth (2019). Open online E-learning resources at epraksis.no as preparation for hands-on laboratory practice. Fulltekst i vitenarkiv
• Sjøholt, Gry; Bjørkhaug, Lise & Ersvær, Elisabeth (2019). E-learning facilitates flipped learning and portofolio assessment in biomedical laboratory science. Fulltekst i vitenarkiv
• Heldal, Ilona; Frøland, Tord Hettervik; Ersvær, Elisabeth & Sjøholt, Gry (2019). mStikk–A Mobile Application for Learning Phlebotomy. Fulltekst i vitenarkiv
• Ersvær, Elisabeth & Sjøholt, Gry (2019). Presentasjon av ePraksis, vinner av KDs utdanningskvalitetspris 2018. Fulltekst i vitenarkiv
• Mæland, Silje; Kristoffersen, Morten; Bovim, Lars Peder Vatshelle; Ersvær, Elisabeth; Harris, Anette & Bjørkhaug, Lise (2019). Handballstjerna håpar ny mensenforsking kan gi svar på kva som gjekk gale. [Journal]. Bergens Tidende . Fulltekst i vitenarkiv
• Frøland, Tord Hettervik; Heldal, Ilona; Ersvær, Elisabeth; Sjøholt, Gry & Kövari, Attila (2019). wStikk – Web-based Phlebotomy Learning. Fulltekst i vitenarkiv Vis sammendrag

  Biomedical Laboratory Science (BLS) students need to learn and train several practical procedures. A web-based virtual reality application, wStikk, was developed to support training and learning of venous blood sampling, an activity that will become a crucial part of their working lives. wStikk aims to facilitate high proficiency learning by playing, using examples, and guidance from associated theories as well as videos from real-life. The aim of this paper is to show hands-on experience from teachers and students about blended learning in phlebotomy and the role of the technologies supporting such learning and training. Data comes from an experimental study where three teachers and six students, three beginners and three experienced in phlebotomy, performed the application twice and were interviewed afterward. Results show a positive attitude towards the use of such an application in the study program, while also pointing out possible pitfalls and improvements for more robust and long-time use.

• Ryningen, Anita; Ersvær, Elisabeth; Bruserud, Øystein & Rundgren, Ida Marie (2017). The effect of pre-analytical factors on circulating monocyte subpopulations. Fulltekst i vitenarkiv
• Ersvær, Elisabeth (2017). Bioingeniørstudenters perspektiver på e-læring som forberedelse innen laboratoriefag: en kvalitativ studie. Fulltekst i vitenarkiv
• Ersvær, Elisabeth (2017). Bioingeniørstudenters oppfatning om e-læring som forberedelse til laboratorieøvelser – www.ePraksis.no. Fulltekst i vitenarkiv
• Ersvær, Elisabeth; Sjøholt, Gry; Iversen, Vegard Vereide & Kristoffersen, Morten (2016). LOW DOSE OF CAFFEINE INGESTION OF 3 MG/KG BODY WEIGHT DOES NOT ENHANCE RESISTANCE EXERCISE PERFORMANCE. Fulltekst i vitenarkiv
• Ersvær, Elisabeth (2016). www.epraksis.no. Fulltekst i vitenarkiv
• Ersvær, Elisabeth (2015). Bioingeniørutdanningen i Bergen. Fulltekst i vitenarkiv
• Ersvær, Elisabeth (2015). e-Læring og omvendt klasserom. Fulltekst i vitenarkiv
• Ersvær, Elisabeth (2015). ePraksis. Digital læringsressurs i medisinsk laboratorieteknologi. Fulltekst i vitenarkiv
• Ersvær, Elisabeth (2014). ePraksis - Forsterket læring i hematologipraksis ved bruk av eLæring. Fulltekst i vitenarkiv
• Ersvær, Elisabeth (2014). E-Praksis – Forsterke læring i hematologipraksis ved bruk av e-læring. Fulltekst i vitenarkiv
• Fredly, Hanne Kristin; Ersvær, Elisabeth & Bruserud, Øystein (2010). PRIMARY HUMAN AML CELLS CAN EXPRESS CALRETICULIN ON THE CELL SURFACE AND RELEASE HEAT SHOCK PROTEIN 70 (HSP70) AND HSP90 DURING APOPTOSIS. Haematologica. ISSN 0390-6078. 95, s. 496–497. Fulltekst i vitenarkiv
• Fredly, Hanne Kristin; Ersvær, Elisabeth & Bruserud, Øystein (2010). PRIMARY HUMAN ACUTE MYELOGENOUS LEUKEMIA CELLS RELEASE HEAT SHOCK PROTEIN (HSP) 70 AND HSP90 DURING APOPTOSIS. Experimental Hematology. ISSN 0301-472X. 38(9), s. S19–S20. Fulltekst i vitenarkiv
• Reikvam, Håkon; Hatfield, Kimberley Joanne; Ersvær, Elisabeth; Ryningen, Anita & Bruserud, Øystein (2010). PHARMACOLOGICAL TARGETING OF THE PI3K-AKT/PKB-MTOR PATHWAY ALTERS LOCAL ANGIOREGULATION IN ACUTE MYELOGENOUS LEUKEMIA. Haematologica. ISSN 0390-6078. 95(suppl 2), s. 266–266. Fulltekst i vitenarkiv
• Olsnes, Astrid Marta; Ersvær, Elisabeth; Ryningen, Anita & Bruserud, Øystein (2008). Chemokine receptor expression by circulating T cells derived from acute leukemia patients with severe chemotherapy-induced cytopenia express a wide range of chemokine receptors. Haematologica. ISSN 0390-6078. 93, s. 456–457. Fulltekst i vitenarkiv
• Olsnes, Astrid Marta; Ersvær, Elisabeth; Ryningen, Anita; Hampson, Peter; Lord, Janet M. & Bruserud, Øystein (2007). The protein kinase C activator PEP005 increases the chemokine release in native acute myelogenous leukemia (AML) blasts. Experimental Hematology. ISSN 0301-472X. 35(9), s. 87–87. Fulltekst i vitenarkiv
• Olsnes, Astrid Marta; Ersvær, Elisabeth & Bruserud, Øystein (2007). In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts: The chemokine release profile of the AML dendritic cells depends on the experimental protocol. Haematologica. ISSN 0390-6078. 92, s. 485–485. Fulltekst i vitenarkiv
• Dyb, Elisabeth; Fossland, Trine; Østergaard, Edvin; Hjelsvold, Rune; Ersvær, Elisabeth & Kubberød, Elin [Vis alle 8 forfattere av denne artikkelen] (2025). Meritteringsordningen i høyere utdanning. Erfaringer fra det første nasjonale symposiet for meritterte undervisere. Norges miljø- og biovitenskapelige universitet (NMBU). ISSN 9788257523183. Fulltekst i vitenarkiv Vis sammendrag

  Den 27. november 2024 ble det første nasjonale symposium for meritterte undervisere i Norge arrangert ved NMBU. Formålet var å skape en arena for dialog og erfaringsutveksling på tvers av institusjonene, for å lære av hverandre og diskutere hvordan meritterte undervisere best kan bidra til økt undervisningskvalitet. <p> <p>Erfaringer fra symposiet viser et tydelig behov for nasjonal samordning for videreutvikling av meritteringsordningen, og for å styrke de merittertes rolle i kvalitetsarbeidet.

• Vågstøl, Unni; Nesse, Hilde; Kvamme, Kjersti; Aschim, Caroline Izabel Martins; Ersvær, Elisabeth & Nygård, Irene [Vis alle 13 forfattere av denne artikkelen] (2021). Utvikling av praksis som læringsarena; Praksislæring. HVL-Bergen. ISSN 9788293677420. Fulltekst i vitenarkiv

Se alle arbeider i NVA