Language: NOR | ENG
2020/2021

2BIO043 Biotechnological Methods

Læringsutbytte

Learning outcomes

Upon successful completion of the course, the student will have achieved the following learning outcomes: 

 

Knowledge:

The student

Cell and Reproductive Biology:

  • has thorough knowledge in scientific theory 
  • has advanced knowledge about relevant techniques in cell and reproductive biotechnologies
  • has knowledge that can be applied in the industry and in further scientific education

Molecular Biology:

  • has an overview of central methods of the following research approaches employed in molecular biology: genomics, functional genomics, transcriptomics and proteomics
  • understands strengths and weaknesses of selected methods related to their successful application in biotechnological research
  • has good understanding of how the different research approaches can complement one another to address questions and/or challenges in applied biotechnology and basic biology

Bioprocess Technology:

  • has knowlegde in the underlying principles of main bioprocess unit operations like fermentation, downstream processing
  • has thorough knowlegde about genetic engineering for recombinant protein expression and production from various cell systems

 

Skills:

The student

Cell and Reproductive Biology:

  • can understand and use scientific literature
  • can write term papers scientifically

Molecular Biology:

  • has thorough experience in writing a term paper, describing methods of one or more of the following approaches with references to concrete examples of their application in biotechnological research: genomics, functional genomics, transcriptomics, proteomics

Bioprocess Technology:

  • understands how lab scale methods transform into large scale, and how main unit operations in downstream processing (centrifugation, filtration, and chromatography) function.
  • has good understanding in design and important features of expression vector system for protein production

 

General competence:

The student

Cell and Reproductive Biology:

  • has thorough knowledge in cell and reproductive biotechnology, the application thereof in industry, in veterinary and human medicine, and in science  
  • can apply skills in R&D questions and the economic implications thereof in the industry
  • has thorough skills and knowledge for working in the industry and taking further scientific education

Molecular Biology:

  • has gained experience presenting course subjects, both theoretical and practical, both orally and in written form, both individually and in a team setting

Bioprocess Technology:

  • has good understanding in the role of bioprocess technology in the production of value added biologicals and biopharmaceuticals
  • has thorough understanding in the main workflow and logic behind bioprocessing both in lab and industrial scale, identifying unit operations and how bioprocessing is employed to increase value of the feed stream 
  • has thorough knowledge of how genetic engineering is used to harness cell systems for protein production

Innhold

Content

Methods in Cell and Reproductive Biology comprises:

  • a lecture-based part providing an overview of special subjects in cell and reproductive biotechnologies
  • one term paper describing selected methods and application thereof 
  • this course will initially discuss examples of biotechnological methods using antibodies and fluorochromes for study of cell biology. This includes theory behind the methods and application thereof. In addition, the spermatogenesis, sperm physiology, sperm traits analyses, fertilization process in the female and assisted reproductive technologies will be covered and discussed

Methods in Molecular Biology comprises:

Introductory, theoretical coverage of central methods employed in:

  • genomics
  • functional genomics
  • transcriptomics
  • proteomics

 

Methods in Bioprocess Technology comprises:

 

  • production of biologicals in the bioprocess/ biopharmaceutical industry
  • fermentation technology
  • microbial and mammalian cell cultures
  • genetic engineering; cloning methods, vector systems, recombinant protein production
  • strategies for protein isolation and separation
  • process economy considerations

Arbeids- og undervisningsformer

Organization and Methods of Instruction

Cell and Reproductive Biology:

  • lectures: Introductory lectures are given to provide an overview of the main themes in the course
  • in-class group activities
  • term paper: A given topic on relevant techniques and application thereof will be discussed by the student
  • Fronter: All relevant course information and scientific articles in the syllabus will be posted on Fronter. In addition, the students will hand in the term paper using Fronter

Molecular Biology:

  • lectures
  • reading assignments
  • group presentations

 

Bioprocess Technology:

  • lectures
  • in-class group activities

Obligatoriske krav som må være godkjent før eksamen kan avlegges

Requirements necessary to take the exam

  • attendance at 100 % of the designated "compulsory" on the lecture Schedule in Reproductive Biology
  • 80 % attendance at all other scheduled lectures
  • a written individual term paper from each of the three main subject areas (Cell and Reproductive Biology, Molecular Biology, Bioprocess Technology) must be passed

Due to the coronavirus pandemic, changes were made in the autumn of 2020/spring of 2021 as follows:

  • attendance at 100 % of the designated "compulsory" on the lecture Schedule in Reproductive Biology
  • a written individual term paper from each of the three main subject areas (Cell and Reproductive Biology, Molecular Biology, Bioprocess Technology) must be passed

 

Eksamen

Assessment

  • an individual oral exam in Cell and Reproductive Biology, counts 40% of the final grade (Autumn semester)
  • an individual four-hour written exam in Molecular Biology, counts 40% of the final grade (Spring semester)
  • an individual two-hour written exam in Bioprocess Technology, counts 20% of the final grade (Spring semester) 

All exams must be passed to receive a final grade.

The exam will be evaluated by letter grade, A-F, where E is the lowest passing grade.

Due to the coronavirus pandemic, changes were made in the autumn of 2020/spring of 2021 as follows:

  • an individual oral exam in Cell and Reproductive Biology, counts 40% of the final grade (Spring semester)
  • an individual six-hour written home exam in Molecular Biology, counts 40% of the final grade (Autumn semester)
  • an individual six-hour written home exam in Bioprocess Technology, counts 20% of the final grade (Autume semester) 

All exams must be passed to receive a final grade.

The exam will be evaluated by letter grade, A-F, where E is the lowest passing grade.