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2020/2021

2BIO035 Bioprocess Technology

Læringsutbytte

Learning outcomes

Upon successful completion of the course, the student will have achieved the following learning outcomes: 

 

Knowledge:

The student

  • has thorough knowledge of the underlying principles of main bioprocess unit operations like fermentation, downstream processing
  • has thorough knowledge about genetic engineering for recombinant protein expression and production from various cell systems
  • has advanced knowledge about factorial experimental set up (Design of Experiments)

 

Skills:

The student  

  • understands how lab scale methods transform into large scale, and how main unit operations in downstream processing (centrifugation, filtration, and chromatography) function
  • has advanced understanding of design of expression vector systems for protein production
  • can perform experimental procedures for recovery and purification of a protein and evaluate the outcome

 

General competence:

The student 

  • understands how and why bioprocessing is employed to increase value of the feed stream
  • has deeper knowledge of the main workflow and logic behind bioprocessing both in lab and industrial scale, and able to identify unit operations
  • can analyze how genetic engineering is used to harness cell systems for protein production

Innhold

Content

  • production of biologicals in the bioprocess/ biopharmaceutical industry
  • fermentation technology
  • microbial and mammalian cell cultures
  • genetic engineering; cloning methods, vector systems, recombinant protein production
  • protein isolation and separation methods; centrifugation, solubility, chromatography and membrane separations
  • process economy considerations
  • structured experimental approuch by Design of Experiments

Arbeids- og undervisningsformer

Organization and Methods of Instruction

  • lectures ca. 20 hours
  • lab exercise - two days
  • in-class group activities

Obligatoriske krav som må være godkjent før eksamen kan avlegges

Requirements necessary to take the exam

  • 100% attendance at the preparatory lab lecture
  • 100% participation at laboratory exercise
  • a minimum of 80% attendance at scheduled lectures

Due to the coronavirus pandemic, changes were made in the autumn of 2020/spring of 2021 as follows:

  • 100% attendance at the preparatory lab lecture
  • 100% participation at laboratory exercise

Eksamen

Assessment

  • an individual lab report counts 60% of the final grade
  • an individual four-hour written exam counts 40% of the final grade

Both exams must be passed to receive a final grade

The exam will be evaluated by letter grade, A-F, where E is the lowest passing grade

Due to the coronavirus pandemic, changes were made in the autumn of 2020/spring of 2021 as follows:

  • an individual theoretical lab report counts 40% of the final grade
  • an individual 24-hour written home exam counts 60% of the final grade

Both exams must be passed to receive a final grade

The exam will be evaluated by letter grade, A-F, where E is the lowest passing grade

Tillatte hjelpemidler til eksamen

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