2017/2018 2BI261 Biochemistry and Molecular Biology lab Læringsutbytte Learning outcomesThe candidate having completed the course have the following learning outcomes:SkillsThe Candidate can master laboratory methods central to the fields of biochemistry and molecular biology, and use of the principles that underlie each method CompetenceThe Candidate can work independently with the techniques and instrumentation employed in the course Innhold Content: Biochemistry: Quantitative measurement, spectrophotometry, isolation and characterisation of DNA and proteins, ion exchange chromatography, SDS-PAGE, enzyme kinetics, carbohydrate chemistry. Molecular Biology: Polymerase chain reaction (PCR), gel electrophoresis, cloning into plasmid vectors, preparation & transformation of competent E. coli, plasmid DNA isolation, restriction analysis, DNA cycle sequencing & capillary electrophoresis, bioinformatics. Arbeids- og undervisningsformer Organisation and Methods of Instruction: group work in the laboratory submission of laboratory reports of completed exercises student presentations independent study groups. Obligatoriske krav som må være godkjent før eksamen kan avlegges Requirements necessary to take the examApproved laboratory reports from all the exercises for which a report is required, 7 in Biochemistry and one in Molecular Biology. Eksamen Assessment individual portfolio (includes two reports from the Biochemistry section, selected by the instructor and one comprehensive report from Molecular Biology section), counts 60 % individual written examination, 4-hour, counts 40 % Both parts must be passed to receive a final grade.The grading system is based on the letters from A-F, where E is the minimum passing grade.